ĐÁNH GIÁ HIỆU QUẢ BỘ SINH PHẨM AMPLISENS SHIG/EIEC-SALM-CAMP-F1 PHÁT HIỆN 3 TÁC NHÂN GÂY BỆNH TIÊU CHẢY BẰNG KỸ THUẬT REAL-TIME PCR
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ASSESSING THE DIAGNOSTIC EFFICACY OF AMPLISENS SHIG / EIEC-SALM-CAMP-F1 KIT IN DETECTION OF THREE BACTERIA CAUSEING INFECTIOUS DIARRHEA BY REAL-TIME PCR TECHNIQUE
Assessing diagnostic efficacy of shig/eiec-salm-camp-f1 (Amplisens) Kit in direction of three bacteria causing cute diarrhea (Campylobacter, Salmonella and Shigella). 96 feces specimens were collected from non-treated pediatric patients with acute diarrhea and 30 controls were collected from normal persons without gastrointestinal disease. The sensitivity and specificity of Amplisen Shig/EIEC-salm-camp-F1 kit (Interlab Servive Company, Russian Federation) were evaluated by using real-time PCR technique. The results showed that Amplisens kit had the high specificity (100%) and high sensitivity (100%). The detection limit was at least 103 CFU/mL. These results were similar to the those of the Sacace kit (standard kit) that has been commercialized in Vietnam. The results also showed that 17.71%, 11.46%, and 7.29 % of the specimens were positive for Campylobacter, Salmonella and Shigella, respectively, in which 1 sample was detected the co-infection of Campylobacter and Salmonella (1.04%). Therefore, the Amplisens kit can be valuable in early diagnosis of acute infectious diarrhea caused by three bacteria including Campylobacter, Shigella, Salmonella in Vietnam.
Từ khóa
Diarrhea, Salmonella, Shigella, Campylobacter, tiêu chảy
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Tài liệu tham khảo
2. Elliott E.J., Acute Gastroenteritis in children, British Medical Journal, 2007, 334:35-40.
3. Sanchez-Capilla A.D., Sorlozano-Puerto A., Rodriguez-Granger J., Martinez-Brocal A., Navarro-Mari J.M., Gutierrez-Fernandez J., Infectious etiology of diarrheas studied in a third-level hospital during a five-year period, Revista Española de Enfermedades Digestivas (The Spanish jounal of Gastroenterology), 2015, 107(2):89-97.
4. Vasco G., Trueba G., Atherton R., Calvopina M., Cevallos W., Andrade T., Eguiguren M., Eisenberg J.N., Identifying etiological agents causing diarrhea in low income Ecuadorian communities, The American Journal of Tropical Medicine and Hygiene, 2014, 91(3):9-563.
5. Nguyễn Thanh Thảo, Lê Thị Tài, Nguyễn Văn Hiến, Lê Thị Hoàn, Nguyễn Hoàng Long, Lê Thị Thanh Xuân, Tình hình bệnh tiêu chảy tại Việt Nam giai đoạn 2002-2011, Tạp chí Y học dự phòng, 2014, Tập XXIV, 7(156):92-96.
6. Trung Vu Nguyen, Phung Le Van, Chinh Le Huy, Khanh Nguyen Gia, Andrej Weintraub, Etiology and epidemiology of diarrhea in children in Hanoi, Vietnam, International Journal of Infectious Diseases, 2006, 10:298-308.
7. Barletta F., Mercado E.H., Lluque A., Ruiz J., Cleary T.G., Ochoa T.J., Multiplex Real-Time PCR for Detection of Campylobacter, Salmonella, and Shigella, Journal of Clinical Microbiology, 2013, 51(9):2822-2829.
8. Ursell L.K., Metcalf J.L., Parfrey L.W. et al., Defining the human microbiome, Nutrition reviews, 2012, 70(1):38-44.
9. Lloyd-Price J., Abu-Ali G., Huttenhower C., The healthy human microbiome, Genome medicine, 2016, 8(1):51.
10. Fereshteh Jafari, Leila Shokrzadeh, Mohammad Hamidian, Siavash Salmanzadeh-Ahrabi, Mohammad Reza Zali, Acute diarrhea due to Enteropathogenic bacteria in patients at hospitals in Tehran, Japanese journal of infectious diseases, Infect. Dis., 2008, 61:269-273.
11. Nguyễn Vũ Trung, Độ nhạy và độ đặc hiệu của PCR đa mồi xác định trực tiếp Salmonella từ bệnh phẩm phân, Y học thực hành, số 1/2009.