ĐẶC ĐIỂM DI TRUYỀN PHÂN TỬ CỦA NEISSERIA MENINGITIDIS (SEROGROUPS VÀ GENOSUBTYPES) PHÂN LẬP Ở MỘT SỐ ĐƠN VỊ QUÂN ĐỘI KHU VỰC PHÍA BẮC (2014-2017)
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Tóm tắt
MOLECULAR CHARACTERIZATION OF NESERRIA MENGINITIDIS SEROGROUPS AND GENOSUBTYPES ISOLATED IN SOME NORTHERN MILITARY UNITS IN THE PERIOD 2012 - 2015
Based on polysaccharide capsule protein, N. meningitidis was subdivided into 13 serogroups. The distribution of serogroups depended on time and geographic areas. Most of these invasive disease cases are strains belonging to serogroups A, B, C, Y, and W-135. PorA is an outer membrane protein of most meningococcal isolates which contains two major variable regions (VR1 and VR2). Determination of VR1 and VR2 regions in porA gene plays very important role in epidemiology examination and vaccine production. Studying the structure of serogroups and genosubtypes of N. meningitidis isolated in several northern military units in period 2014 - 2017 showed that the serogroup B contributed to the highest rate which was estimated in 79.17%, followed serogroup C (6.25%), and the remaining rates belongs to the other serogroups except serogroups A, B, C, W135, and Y. The results for determination of genosubtype based on VR1 and VR2 regions of porA gene in 38 strains indicated that the highest genosubtypes were P1.22-25,14 (34,2%), followed P1.7-2,13-1 (10,5%). Other genosubtypes accounted from 2,6% to 7,9%. 7 strains (18,4%) were non-genosubtypeable.
Từ khóa
Não mô cầu, nhóm huyết thanh, porA, phân týp huyết thanh, Neisseria meningitidis, serogroup, serosubtype
Chi tiết bài viết
Tài liệu tham khảo
2. Quỳnh Viên, Viêm màng não do não mô cầu, Tạp chí Thuốc và Sức khỏe, 2014, 4:24-25.
3. Triệu Phi Long, Lê Thu Hà, Lê Hải Yến, Đoàn Trọng Tuyên, Giám sát dịch tễ học não mô cầu tại một số đơn vị quân đội khu vực miền Bắc từ 2008- 2014, Tạp chí Truyền nhiễm Việt Nam, 2015, 2:16-21.
4. Barra G.N., Araya P.A., Fernandez J.O., Gabastou J.M., Hormazábal J.C., Seoane M., Pidal P.C., Valenzuela M.T., Ibarz-Pavón A.B., Molecular characterization of invasive Neisseria meningitidis strains isolated in Chile during 2010-2011, PLoS One, 2013, 8(6):e66006. doi: 10.1371/journal.pone.0066006.
5. Boan P., Metasan N., Tempone S., Harnett G., Speers D.J., Keil A.D., Neisseria meningitidis porA, fetA and fHbp gene distribution in Western Australia 2000 to 2011, BMC Infect Dis. 2014, 14: doi: 10.1186/s12879-014-0686-x.
6. Diggle M.A., Clarke S.C., Detection and genotyping of meningococci using a nested PCR approach, Journal of Medical Microbiology, 2003, 52:51-57.
7. Jelfs J., Munro R., Wedege E., Caugant D.A., Sequence variation in the porA gene of a clone of Neisseria meningitidis during epidemic spread, Clin Diagn Lab Immunol., 2000, 7(3):390-395.
8. Khatami A., Pollard Ạ., The epidemiology of meningococcal disease and the impact of vaccines, Expert Rev. Vaccines, 2010, 9(3):285-298.
9. Soeters H.M., Dinitz-Sklar J., Kulkarni P.A., MacNeil J.R., McNamara L.A., Zaremski E., Montana B., Serogroup B Meningococcal Disease Vaccine Recommendations at a University, New Jersey, USA, 2016, Emerging Infectious Diseases, 2017, 23(5):867-869,
10. Taha M.K., Simultaneous approach for nonculture PCR-based identification and serogroup prediction of Neisseria meningitidis, J Clin Microbiol., 2000, 38(2):855-857.
11. Vicente D., Esnal O., Michaus L., López de Goicoechea M.J., Cisterna R., Pérez-Trallero E., Prevalence of genosubtypes (PorA types) of serogroup B invasive meningococcus in the north of Spain from 2000 to 2003, J Med Microbiol., 2005, 54(4):381-384.