PHÁT HIỆN GENE BẢN THỂ OXA-51-LIKE CỦA Acinetobacter baumannii BẰNG PHƯƠNG PHÁP REAL-TIME PCR
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Tóm tắt
DETECTION OF INTRINSIC OXA-51-LIKE GENE IN Acinetobacter baumannii BY REAL-TIME PCR METHOD
Acinetobacter baumannii is one of leading causes of nosocomial infection. The bacteria possess instrinsic OXA-51-like gene. Moreover, with this gene the bacteria could resist to many antibiotics.The study aims to develop a real-time PCR protocol to detect intrinsic OXA-51-like gene in A. baumannii. The real-time PCR has been optimized with DNA-binding dye (EvaGreen) for amplifying OXA-51-like gene specific to A. baumannii and 16S rRNA gene specific to Acinetobacter spp. in one reaction. The protocol after setup was applied with several A. baumannii DNA samples. The result shows that the real-time PCR protocol can correctly detect intrinsic OXA-51-like gene in A. baumannii with the sensitivity of 32 copies/reaction and high specificity. The ratio of A. baumannii detection reached 100% for DNA samples which A. baumannii had been detected. The real-time PCR protocol using EvaGreen to detect OXA-51-like gene in A. baumannii has been developed successfully. The protocol can be used to rapidly detect A. baumannii based on this gene.
Từ khóa
A. baumannii, carbapenemase, OXA-51, EvaGreen, Real-time PCR
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Tài liệu tham khảo
2. Nguyễn Tuấn Anh, Huỳnh Minh Tuấn, Nguyễn Văn Vĩnh Châu, Hồ Huỳnh Thùy Dương. Xây dựng quy trình EvaGreen real-time PCR phát hiện các gen blaOXA ở Acinetobacter baumannii, Tạp chí Y học Thành phố, 2014, 18(5):197-201.
3. Peleg A.Y., Seifert H., Paterson D.L., Acinetobacter baumannii: emergence of a successful pathogen, Clin Microbiol Rev, 2008, 21(3):82-538.
4. Theaker C., Azadian B., Soni N., The impact of Acinetobacter baumannii in the intensive care unit, Anaesthesia, 2003, 58(3):4-271.
5. Ecker et al., Identification of Acinetobacter species and genotyping of Acinetobacter baumannii by multilocus PCR and mass spectrometry, J Clin Microbio., 2006, l44(8):32-2921.
6. McConnell et al., Quantitative real-time PCR for detection of Acinetobacter baumannii colonization in the hospital environment, J Clin Microbio., 2012, 150(4):4-1412.
7. De Gregorio et al., Development of a real-time PCR assay for the rapid detection of Acinetobacter baumannii from whole blood samples, New Microbiol, 2015, 38(2):7-251.
8. Huang et al., Development and validation of a multiplex TaqMan real-time PCR for rapid detection of genes encoding four types of class D carbapenemase in Acinetobacter baumannii, J Med Microbio., 2012, 161(11):7-1532.