PHÂN LẬP VÀ TUYỂN CHỌN VI KHUẨN ĐẤT CÓ KHẢ NĂNG PHÂN HỦY THUỐC BẢO VỆ THỰC VẬT CƠ PHOSPHO
Nội dung chính của bài viết
Tóm tắt
ISOLATION AND SCREENING OF SOIL BACTERIA CAPABLE OF DEGRADING SOME ORGANOPHOSPHORUS PESTICIDES
For many decades, organophosphorus compounds (OPs) have been widely used all over the world for several purposes like chemical weapons, flame-retardants, ectoparasiticides and investigational new drugs, but mainly used in agriculture and indoors. However, they have high toxicity to animals and also to humans. Various methods have been used to detoxify these compounds. Among them, biodegradation is preferable, as this method is environmentally and produces less harmful substances. In this study, from the 55 soil samples collected from many places where pesticides were used, we isolated and obtained 9 bacterial strains capable of degrading OPs, of which 5 strains (OP9, OP10, OP11, OP13, OP15) are capable of using all three types of OPs (methyl parathion, diazinon, chlorpyrifos) as the sole carbon source. The OP15 strain has been identified as Sphingobium sp. OP15, and exhibited a remarkable diazinon degradability in the minimal medium with a reduction of 93.14% after 14 days of of growing (initial diazinon concentration 50 mg/l). Research results open the possibility of applying these bacterial strains in OPs contaminated soil treatment.
Từ khóa
Organophosphorus, biodegradation, TLC, GC-MS, phân hủy sinh học, hợp chất cơ phospho
Chi tiết bài viết
Tài liệu tham khảo
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