XÁC ĐỊNH PARACETAMOL TRONG MẪU DƯỢC PHẨM BẰNG PHƯƠNG PHÁP PHỔ HẤP THỤ PHÂN TỬ UV-VIS SỬ DỤNG HIỆN TƯỢNG CỘNG HƯỞNG PLASMON BỀ MẶT CỦA DUNG DỊCH NANO VÀNG
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Tóm tắt
DETERMINATION OF PARACETAMOL IN PHARMACEUTICAL SAMPLES BY UV-VIS SPECTROPHOTOMETRIC METHOD USING SURFACE PLASMON RESONANCE (SPR) OF NANO GOLD SOLUTION
A highly sensitive molecular absorption UV-VIS method has been developed for the measurement of paracetamol in drug samples, based on the change in surface plasmon resonance (SPR) properties of gold nano particles in the presence of paracetamol. Visual detection of paracetamol trace in nano gold particle solution is possible through color change of solution from red to blue. The optical absorption ratio A690/A520 increases linearly with logarithm of paracetamol concentration in the range 5×10-7 M - 2.5×10-5 M. At the optimum conditions including 0.038 M NaCl, pH 4 with incubation time of 17 minutes, the developed method has very low limit of detection (LOD) of 1.61×10-7 M, relative standard deviation %RSD < 5%. The influence of possible coexisting subtances (Na2CO3, caffeine, dextromethorphan) in the drug samples is negligible (%S < 6%). The colorimetric method was successfully applied for the determination of paracetamol in serveral drug samples.
Từ khóa
Paracetamol, Au nano particles, spectrophotometry, UV-Vis, hạt nano Au, quang phổ
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Tài liệu tham khảo
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