COMPARATIVE ANALYSIS OF THE CHRONIC VIRAL HEPATITIS B MARKERS PREVALENCE IN HIV-INFECTED AND CONDITIONALLY HEALTHY RESIDENTS OF HO CHI MINH CITY
Saint-Petersburg Pasteur Institute (Saint Petersburg, Russia)
Head of the Laboratory of Virology and Immunology HIV, Senior Researcher at the Laboratory of Molecular Immunology St. Petersburg Pasteur Institute. Russian Federation,197101, St. Petersburg, st. Mira, 14.
Số điện thoại: +7 (812) 233-20-92 +7-921-353-81-73; Email: ostankova@pasteurorg.ru
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Tóm tắt
COMPARATIVE ANALYSIS OF THE CHRONIC VIRAL HEPATITIS B MARKERS PREVALENCE IN HIV-INFECTED AND CONDITIONALLY HEALTHY RESIDENTS OF HO CHI MINH CITY
The prevalence of chronic HBV infection, as measured by the hepatitis B surface antigen (HBsAg) prevalence, is 8-20% in the general population and 31-54% in the high-risk urban population in Vietnam. Vietnam is currently one of the countries with the highest mortality rates from liver cancer associated mainly with HBV infections. One of CHB natural progression forms is presented by occult hepatitis B infection (OBI), which is characterized by HBV DNA persistence in liver tissue and extremely low HBV DNA concentration with undetectable HBsAg level in peripheral blood. One of the tasks required is to estimate the prevalence of CHB and OBI among conventionally healthy people and in risk groups (for example, among HIV-infected individuals). The analysis of the overall prevalence of serological markers showed that among the conditionally healthy individuals and HIV-infected persons HBsAg were detected in 12.3% and 9.49% of individuals, respectively. The prevalence of HBV DNA in the examined groups (26.95% and 32.58%) exceeds the previously published prevalence rates for the virus in the population in region. Our results of HBV genotyping indicate that HBV genotype B dominates in residents of Ho Chi Minh City, followed by genotype C. However, HBV genotype B occurrence frequency among HBsAg-positive individuals is much higher, than the corresponding frequency among OBI patients. In addition, in our study, a significant number of C5 subtype samples (3.74% conditionally healthy persons, 4.34% HIV-infected individuals) were identified among HBsAg-negative samples. The results obtained in this work demonstrate a high prevalence of CHB/OBI in the region and indicate the need for further large-scale studies of HBV in order to improve strategies for disease control and prevention in the Vietnam.
Từ khóa
HBV, markers of viral hepatitis, molecular epidemiology, subtype, conditionally healthy residents, HIV-infected individuals, coinfection, Ho Chi Minh City
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Tài liệu tham khảo
2. World Health Organization, Hepatitis B. Key facts, 2022, Available at: http://www.who.int/news-room/fact-sheets/detail/hepatitis-b Access date: 14.06.2022.
3. World Health Organization Prevention and control of viral hepatitis infection: frame work for global action 2012, Geneva: WHO, 2012, Available at: https://apps.who.int/iris/handle/10665/130012 Access date: 14.06.2022.
4. Raimondo G., Locarnini S., Pollicino T., Levrero M., Zoulim F., Anna S. Lok, Taormina workshop on occult HBV infection faculty members. Update of the statements on biology and clinical impact of occult hepatitis B virus infection, J. Hepatol., 2019, 71(2):397-408.
5. Maldonado-Rodriguez A., Cevallos A. M., Rojas-Montes O., Enriquez-Navarro K., Alvarez-Muñoz M. T., Lira R., Occult hepatitis B virus co-infection in human immunodeficiency virus-positive patients: A review of prevalence, diagnosis and clinical significance, World J. Hepatol., 2015, 7(2):253-60.
6. Gish R. G., Bui T. D., Nguyen C. T., Nguyen D. T., Tran H. V., Diem M. T. Tran, Huy N. Trinh, International Group for Liver Health in Viet Nam, Liver disease in Viet Nam: screening, surveillance, management and education: a 5-year plan and call to action, J. Gastroenterol. Hepatol., 2012, 27(2):238-247.
7. Do S. H., et al., High prevalences of hepatitis B and C virus infections among adults living in Binh Thuan province, Vietnam, Hepatol. Res., 2015, 45(3):259-268.
8. Ostankova Yu. V., Semenov A. V., Totolian Areg A., Hepatitis B virus identification in a blood plasma at a low viral load, Klinicheskaya Laboratornaya Diagnostika (Russian Clinical Laboratory Diagnostics), 2019, 64(10):635-640.
9. Ostankova Yu. V., Serikova E. N., Semenov A. V., Totolian Areg A., Method for hepatitis B virus DNA detecting in biological material at low viral load based on nested PCR with detection on three viral targets in real-time mode, Klinicheskaya Laboratornaya Diagnostika (Russian Clinical Laboratory Diagnostics), 2022, 67(9):530-537 (in Russ.).
10. Boumbaly S., Serikova E. N., Balde T. A. L., Ostankova Yu. V., Schemelev A. N., Valutite D. E., Zueva E. B., Semenov A. V., Totolian Areg A., Amino acid substitutions in core and HBsAg regions of hepatitis B virus in patients with monoinfection and HBV/HIV-coinfection in the Republic of Guinea, HIV Infection and Immunosuppressive Disorders, 2021, 13(3):122-133.
11. Tamura K., Stecher G., Kumar S., MEGA11: Molecular evolutionary genetics analysis version 11, Mol. Biol. Evol., 2021, 38(7):3022-3027.
12. Trang N. H., et al., Molecular characteristics of hepatitis B virus (HBV) isolated from chronic hepatitis B patients in South Vietnam, Virus Evol., 2017, 3(Suppl 1). pii: vew036.016.
13. Flower B., Du Hong D., Vu Thi Kim H., Pham Minh K., Geskus R. B., Day J., Cooke G. S., Seroprevalence of hepatitis B, C and D in Vietnam: A systematic review and meta-analysis, Lancet Reg. Health West Pac., 2022, 24:100468.
14. Kishk R., Atta H. A., Ragheb M., Kamel M., Metwally L., Nemr N., Genotype characterization of occult hepatitis B virus strains among Egyptian chronic hepatitis C patients, East. Mediterr. Health. J., 2014, 20(2):130-138.
15. Schweitzer A., Horn J., Mikolajczyk R. T., Krause G., Ott J. J., Estimations of worldwide prevalence of chronic hepatitis B virus infection: a systematic review of data published between 1965 and 2013, The Lancet, 2017, 386(10003):1546-1555.
16. Truong B. X., Seo Y., Yano Y., Ho P. T., Phuong T. M., Long D. V., Son N. T., Long N. C., Kato H., Hayashi Y., Trach N. K., Kasuga M., Genotype and variations in core promoter and pre-core regions are related to progression of disease in HBV-infected patients from Northern Vietnam, Int. J. Mol. Med., 2007, 19(2):293-299.