VIRUS-LIKE IMMUNOSTIMULATORY COMPLEXES BASED ON PLANT SAPONINS: PREPARATION, QUALITY CONTROL, ADJUVANT PROPERTIES
State Research Center for Virology and Biotechnology “Vector”, Federal Service for Surveillance of Consumer Rights Protection and Human Welfare
12a, Koltsovo settlement, Novosibirsk Region, 630559, Russian Federation
Số điện thoại: +79529026752; Email: evseenko_va@vector.nsc.ru
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Tóm tắt
A promising approach to immunization is the use of saponin-based virus immunostimulatory complexes (ISCOM) and the creation of antigen complexes (ISCOM-antigen) based on them. Objective: production and study of virus-like immunostimulatory complexes based on Quillaja saponaria saponins. Materials and methods: using the tangential filtration method, the preparations of ISCOM adjuvant “Matrix-V”, were obtained. Electron microscopic study of the preparations confirmed expected object ultrastructure. The content of saponins, residual detergent in the preparation controlled by HPLC method. Immunization of guinea pigs, Balb/c mice with preparations of ISCOM-antigen (influenza virus A, B; Covid-19) performed intramuscularly. Blood serum samples of immunized animals examined in hemagglutination inhibition reaction, neutralization assay. Results: ISCOM containing Quillaja saponaria saponins were successfully qualified. Concentrations of saponins and residual detergent in the obtained preparations determined and were 47.9 mg/ml and 0.63 mg/ml respectively. As a result of a double intramuscular immunization of mice with 50 ng of hemagglutinin of each subtype, GMT in HI in serum was 1:735 - H1N1pdm09; 1:320 - H3N2; 1:91 - B(Yamagata); 1:60 - B(Victoria). No immune response to the vaccine in the same concentration without ISCOM was detected in HI. When immunized with Covid-19 antigens, virus neutralization titer was 1:20, with the Covid-19-ISCOM-adjuvant complex 1:1114 - 1:1436. The presented results indicate the promising use of ISCOM-based preparations in the development of vaccines.
Từ khóa
Virus-like immunostimulatory complexes, ISCOM, adjuvant, saponin, vaccine, immunization, influenza, Covid-19, respiratory infection
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Tài liệu tham khảo
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